Department of Materials Science and Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China.
J Biomed Mater Res A. 2011 Nov;99(2):158-65. doi: 10.1002/jbm.a.33090. Epub 2011 Aug 16.
A dense poly-L-lactic acid (PLLA) film was employed as the primary material and hot-embossed with the formation of microgrooves (g-PLLA). A thin layer of Au was then deposited on the film to obtain a morphologically modified substrate (Au/g-PLLA). The Au/g-PLLA film surface was then chemically modified by imprinting octadecanethiolate (ODT) self-assembled monolayers on the upper surface (ODT/Au/g-PLLA), followed by Arg-Gly-Asp (RGD) peptide sequences on the microgrooves (RGD_ODT/Au/g-PLLA). The surface chemistry of the as-prepared RGD_ODT/Au/g-PLLA samples was examined. The bioactivity and spreading function of Schwann cells cultured on the morphologically and chemically modified surfaces were assessed. The results demonstrate that Schwann cells adhered to the RGD/Au/g-PLLA surface and proliferated along the microgrooves without crossing over the ODT/Au/PLLA surface. The proposed film surface can be used for manipulating the outgrowth of axons by modifying and arranging a selected region to induce cell growth and to prevent cells from spreading out nondirectionally.
采用聚 L-乳酸（PLLA）薄膜作为主要材料，通过热压形成微槽（g-PLLA）。在薄膜上沉积一层薄薄的金，得到形态修饰的基底（Au/g-PLLA）。Au/g-PLLA 薄膜表面通过在其表面化学印记十八烷硫醇自组装单层（ODT/Au/g-PLLA）进行化学修饰，然后在微槽上修饰 Arg-Gly-Asp（RGD）肽序列（RGD_ODT/Au/g-PLLA）。对制备的 RGD_ODT/Au/g-PLLA 样品的表面化学性质进行了研究。评估了 Schwann 细胞在形态和化学修饰表面上的生物活性和扩展功能。结果表明，Schwann 细胞附着在 RGD/Au/g-PLLA 表面上，并沿着微槽生长，而不会越过 ODT/Au/PLLA 表面。该薄膜表面可用于通过修饰和排列选定区域来操纵轴突的生长，以诱导细胞生长并防止细胞无方向扩散。
