National Engineering Laboratory for Carbon Fiber Technology, Institute of Coal Chemistry, Chinease Academy of Sciences, Taiyuan 030001, [corrected] People's Republic of China.
J Biomed Mater Res A. 2011 Nov;99(2):166-72. doi: 10.1002/jbm.a.33134. Epub 2011 Aug 16.
Magnesium and its alloys have been used in the recent development of lightweight, biodegradeable implant materials. However, the corrosion properties of magnesium limit its usefulness. In a previous study, a micro-arc oxidation (MAO) method was used to modify a Mg-1.0 wt % Zn-1.0 wt % Ca alloy surface, with the purpose of improving the corrosion resistance of Mg alloys. However, the blood compatibility of MAO-treated Mg alloy is unknown. Results of cytotoxicity assays with bone marrow-derived mesenchymal stem cells showed that extracts of MAO-treated alloy significantly decreased cytotoxicity compared to titanium alloy extract. Results of blood compatibility tests showed that the MAO group had a decreased hemolytic ratio (2.25%) compared to the untreated Mg alloy group (24.58%) (p < 0.001). The MAO group showed significantly shorter prothrombin and thrombin times and significantly longer activated partial thromboplastin time than the untreated Mg alloy group. Arachidonic acid- and adenosine diphosphate-induced platelet aggregations were significantly decreased by the untreated Mg alloy extract, and they were less affected by extract of MAO-treated Mg alloy. In conclusion, MAO-treated Mg-1.0 wt % Zn-1.0 wt % Ca alloy exhibits favorable blood compatibility characteristics and may be useful in the development of magnesium implant materials.
镁及其合金已被用于开发新型轻量、可生物降解的植入材料。然而，镁的腐蚀性能限制了它的用途。在之前的研究中，采用微弧氧化（MAO）方法对 Mg-1.0wt%Zn-1.0wt%Ca 合金表面进行了改性，目的是提高镁合金的耐腐蚀性。然而，MAO 处理后的镁合金的血液相容性尚不清楚。骨髓间充质干细胞的细胞毒性试验结果表明，与钛合金浸提液相比，MAO 处理后的合金浸提液显著降低了细胞毒性。血液相容性试验结果表明，与未经处理的镁合金组（24.58%）相比，MAO 组的溶血率（2.25%）降低（p<0.001）。MAO 组的凝血酶原时间和凝血酶时间明显缩短，而活化部分凝血活酶时间明显延长，与未经处理的镁合金组相比。未经处理的镁合金浸提液可显著降低花生四烯酸和二磷酸腺苷诱导的血小板聚集，而 MAO 处理后的镁合金浸提液的影响较小。总之，MAO 处理后的 Mg-1.0wt%Zn-1.0wt%Ca 合金具有良好的血液相容性，可能有助于开发镁植入材料。
