Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.
J Biomed Mater Res A. 2011 Nov;99(2):192-202. doi: 10.1002/jbm.a.33175. Epub 2011 Aug 16.
Nanocomposites from a polyether-type waterborne polyurethane (PU) and 0.1 wt % of silicate materials were prepared. The individual silicate materials were natural clays (montmorillonite and mica), their exfoliated clays [nano silicate platelets (NSP) and nano mica platelets], and NSP modified with C18 fatty amine (NSP-S). The physico-chemcical properties and antimicrobial activity of the nanocomposites were characterized in vitro. The biostability and biocompatibility of the nanocomposites were evaluated in vivo. The nanocomposites exhibited various surface morphologies with phase separation of hard and soft domains in nanometric scales. The nanocomposite containing NSP (PU-NSP) showed better endothelial cell attachment and gene expression. The better biocompatibility of PU-NSP and PU-NSP-S was evidenced by the lower thickness of foreign body capsules in rat subcutaneous implantation. PU-NSP had the least surface degradation in vivo as demonstrated by the electron microscopy and infrared spectroscopy. This may be associated with the different surface structure. PU-NSP and PU-NSP-S showed strong bacteriostatic effects, which suggested that the nano clay in the polymer matrix may still interact with the microbes.
采用聚醚型水性聚氨酯（PU）和 0.1wt%的硅酸盐材料制备纳米复合材料。单独的硅酸盐材料为天然粘土（蒙脱石和云母）、其剥离粘土[纳米硅酸盐片层（NSP）和纳米云母片层]和用 C18 脂肪酸胺改性的 NSP（NSP-S）。体外对纳米复合材料的物理化学性质和抗菌活性进行了表征。体内评估了纳米复合材料的生物稳定性和生物相容性。纳米复合材料表现出不同的表面形态，具有纳米级的硬软域相分离。含有 NSP 的纳米复合材料（PU-NSP）显示出更好的内皮细胞附着和基因表达。在大鼠皮下植入中，具有较低的异物囊厚度，表明 PU-NSP 和 PU-NSP-S 具有更好的生物相容性。通过电子显微镜和红外光谱证明，PU-NSP 在体内具有最小的表面降解。这可能与不同的表面结构有关。PU-NSP 和 PU-NSP-S 表现出很强的抑菌作用，这表明聚合物基质中的纳米粘土可能仍与微生物相互作用。
