[Construction and recovery of chimeric rabies virus expressing envelop proteins E1E2 of hepatitis C].
作者信息
Du Jia-Liang, Huang Ying, Tang Qing, Wang Li-Hua, Liang Guo-Dong
机构信息
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Jun;25(3):170-2.
OBJECTIVE
Construction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2.
METHODS
On the basis of the previously established reverse genetic system CTN-GFP, HCV E1E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNdeltaG-HCV E1E2 were recovered.
RESULTS
The result demonstrated that both the chimeric viruses were rescued successfully, had the ability to re-infect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA.
CONCLUSION
The establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.
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