State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, China.
Anal Chem. 2011 Nov 15;83(22):8787-93. doi: 10.1021/ac2023537. Epub 2011 Oct 27.
While the super fluorescence quenching capacity of graphene and graphene oxide (GO) has been extensively employed to develop fluorescent sensors, their own unique fluorescence and its potential for chemo-/biosensing have seldom been explored. Here we report a GO-based photoinduced charge transfer (PCT) label-free near-infrared (near-IR) fluorescent biosensor for dopamine (DA). The multiple noncovalent interactions between GO and DA and the ultrafast decay at the picosecond range of the near-IR fluorescence of GO resulted in effective self-assembly of DA molecules on the surface of GO, and significant fluorescence quenching, allowing development of a PCT-based biosensor with direct readout of the near-IR fluorescence of GO for selective and sensitive detection of DA. The developed method gave a detection limit of 94 nM and a relative standard deviation of 2.0% for 11 replicate detections of 2.0 μM DA and was successfully applied to the determination of DA in biological fluids with quantitative recovery (98-115%).
虽然石墨烯和氧化石墨烯（GO）的超荧光猝灭能力已被广泛用于开发荧光传感器，但它们自身独特的荧光及其用于化学/生物传感的潜力很少被探索。在这里，我们报告了一种基于 GO 的光诱导电荷转移（PCT）无标记近红外（近红外）荧光生物传感器，用于检测多巴胺（DA）。GO 与 DA 之间的多种非共价相互作用以及 GO 近红外荧光的皮秒级超快衰减导致 DA 分子在 GO 表面有效自组装，并发生显著荧光猝灭，从而实现了基于 PCT 的生物传感器的发展，可直接读取 GO 的近红外荧光，用于选择性和灵敏地检测 DA。所开发的方法对 2.0 μM DA 的 11 个重复检测的检测限为 94 nM，相对标准偏差为 2.0%，并成功应用于生物体液中 DA 的定量测定（回收率为 98-115%）。
