Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Int J Pharm. 2011 Dec 12;421(1):183-8. doi: 10.1016/j.ijpharm.2011.09.029. Epub 2011 Sep 29.
Gene therapy targeted at the respiratory epithelium holds therapeutic potential for diseases such as cystic fibrosis and lung cancer. We recently reported that Chitosan-DNA-FAP-B nanoparticles are good candidates for targeted gene delivery to fibronectin molecules (FAP-B receptors) of lung epithelial cell membrane. In this study Chitosan-DNA-FAP-B nanoparticles were nebulized to mice using air jet nebulizer. The effect of nebulization on size, zeta potential and DNA binding ability of nanoparticles were studied. The level of gene expression in the mice lungs was evaluated. Nebulization did not affect the physicochemical properties of nanoparticles. Aerosol delivery of Chitosan-DNA-FAP-B nanoparticles resulted in 16-fold increase of gene expression in the mice lungs compared with Chitosan-DNA nanoparticles. This study suggested that Chitosan-FAP-B nanoparticle can be a promising carrier for targeted gene delivery to the lung.
针对呼吸道上皮细胞的基因治疗为囊性纤维化和肺癌等疾病提供了治疗潜力。我们最近报道称，壳聚糖-DNA-FAP-B 纳米粒是针对肺上皮细胞膜上纤维连接蛋白分子（FAP-B 受体）靶向基因传递的良好候选物。在这项研究中，我们使用空气射流式雾化器将壳聚糖-DNA-FAP-B 纳米粒雾化给小鼠。研究了雾化对纳米粒大小、zeta 电位和 DNA 结合能力的影响。评估了小鼠肺部的基因表达水平。雾化处理不会影响纳米粒的理化性质。与壳聚糖-DNA 纳米粒相比，壳聚糖-FAP-B 纳米粒的气溶胶传递使小鼠肺部的基因表达增加了 16 倍。这项研究表明，壳聚糖-FAP-B 纳米粒可能是一种有前途的肺部靶向基因传递载体。
