Division of Pharmacotherapeutics, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, 86 Nishicho, Yonago 683-8503, Japan.
J Pharmacol Sci. 2011;117(3):129-38. doi: 10.1254/jphs.11097fp. Epub 2011 Oct 7.
The protective effects of hesperidin against hypercholesterolemia and fatty liver were examined in male Wistar rats fed a high-cholesterol diet for 12 weeks. Compared with a standard diet, a high-cholesterol diet not only increased body weights, liver weights, and serum concentration of cholesterol, but also induced the fatty degeneration (steatosis) of liver. Hesperidin (0.08%) reduced levels of hepatic steatosis, adipose tissue and liver weights (P < 0.05), serum total cholesterol and retinol binding protein (RBP) 4 concentrations (P < 0.05) in rats fed with high-cholesterol diet, while reduction in low-density lipoprotein cholesterol levels and triglyceride concentrations was not significant. It also attenuated the marked changes in mRNA expression of lipid metabolism-related proteins: RBP, heart fatty acid-binding protein (H-FABP), and cutaneous fatty acid-binding protein (C-FABP), in liver and adipose tissue. According to the results of gas chromatography, serum concentrations of total cholesterol and biomarkers of cholesterol synthesis (lathosterol) and absorption (campesterol, β-sitosterol) were lower, and concentrations of cholesterol in feces were higher in the rats given hesperidin (P < 0.05). Hesperidin may improve hypercholesterolemia and fatty liver by inhibiting both the synthesis and absorption of cholesterol and regulating the expression of mRNA for RBP, C-FABP, and H-FABP.
橙皮苷对高胆固醇血症和脂肪肝的保护作用在 12 周内给予高胆固醇饮食的雄性 Wistar 大鼠中进行了研究。与标准饮食相比，高胆固醇饮食不仅增加了体重、肝脏重量和血清胆固醇浓度，还诱导了肝脏脂肪变性（脂肪变性）。橙皮苷（0.08%）降低了高胆固醇饮食喂养大鼠的肝脂肪变性、脂肪组织和肝脏重量（P<0.05）、血清总胆固醇和视黄醇结合蛋白 4 浓度（P<0.05），而低密度脂蛋白胆固醇水平和甘油三酯浓度的降低则不显著。它还减弱了肝脏和脂肪组织中与脂质代谢相关的蛋白质的 mRNA 表达的明显变化：RBP、心脏脂肪酸结合蛋白（H-FABP）和皮肤脂肪酸结合蛋白（C-FABP）。根据气相色谱的结果，血清总胆固醇和胆固醇合成标志物（羊毛甾醇）和吸收标志物（菜油固醇、β-谷固醇）的浓度较低，给予橙皮苷的大鼠粪便中的胆固醇浓度较高（P<0.05）。橙皮苷可能通过抑制胆固醇的合成和吸收并调节 RBP、C-FABP 和 H-FABP 的 mRNA 表达来改善高胆固醇血症和脂肪肝。
