Department of Pediatrics, Penn State College of Medicine, MC-H085, The Milton S. Hershey Medical Center, PO Box 850, 500 University Drive, Hershey, PA 17033-0850, USA.
Pediatr Clin North Am. 2011 Oct;58(5):1201-15, x. doi: 10.1016/j.pcl.2011.07.011.
Whether for the prepubertal or pubertal child, the goal of fertility preservation is to obtain cells or tissues to be used to produce future children. For the prepubertal child, preservation efforts involve germ cells, earlier forms of sperm, and immature follicles, rather than mature spermatozoa or follicles. Options for prepubertal children include for boys freezing testicular tissue and extracting testicular sperm or for girls obtaining ovarian cortical or follicular tissue for storage. These procedures involve extraction and storage of immature gametes for subsequent in vitro maturation, although attempts for sperm currently involve only animal studies. For adolescent subjects who have sufficient gonadal development and reserve, sperm, oocytes, and ovarian cortex can be retrieved as among adults.
无论是对于青春期前还是青春期的儿童，生育力保存的目标都是获取可用于未来生育的细胞或组织。对于青春期前的儿童，保存工作涉及生殖细胞、更早形式的精子和未成熟的卵泡，而不是成熟的精子或卵泡。青春期前儿童的选择包括男孩冷冻睾丸组织并提取睾丸精子，或女孩获取卵巢皮质或卵泡组织进行储存。这些程序涉及提取和储存未成熟的配子，以便随后进行体外成熟，尽管目前精子的尝试仅涉及动物研究。对于有足够性腺发育和储备的青少年，可像成年人一样获取精子、卵子和卵巢皮质。
