State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Fertil Steril. 2011 Dec;96(6):1479-84. doi: 10.1016/j.fertnstert.2011.09.022. Epub 2011 Oct 6.
To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring.
Randomized research experimental study.
Academic basic research laboratory.
ANIMAL(S): Mice.
INTERVENTION(S): Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring.
MAIN OUTCOME MEASURE(S): Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19.
RESULT(S): Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3.
CONCLUSION(S): Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring.
研究母亲卵母细胞排卵后老化是否会影响后代卵母细胞印迹基因的 DNA 甲基化获得。
随机研究实验。
学术基础研究实验室。
老鼠。
将新鲜卵母细胞和老化卵母细胞从母亲体内人工授精，并从所得后代中收集卵母细胞。
评估母系印迹基因 Peg3、Snrpn、Peg1 和父系印迹基因 H19 卵母细胞中差异甲基化区域（DMR）的甲基化状态。
我们的结果表明，来自老化卵母细胞后代卵母细胞中 Peg3、Snrpn、Peg1 和 H19 的 DMR 上的甲基化模式主要是正常的，只有少数卵母细胞在 Peg3 的 DMR 上表现出异常甲基化。
排卵后卵母细胞老化会降低生殖结局，但不会明显导致有活力后代卵母细胞中 DNA 甲基化印迹获得的缺陷。
