Zambruno G, Santantonio M L, Giannetti A
Clinica Dermatologica, Università di Modena.
G Ital Dermatol Venereol. 1990 Mar;125(3):59-77.
In the last fifteen years various epidermal cell (EC) culture systems have been developed suitable both for investigation purposes and for clinical applications. In organ and explant cultures skin pieces are grown to obtain in the first case vertical proliferation of epidermis and in the second outward migration and proliferation of EC. In isolated EC cultures, EC suspensions are seeded on plastic or specific substrates (collagens, fibronectin, 3T3 fibroblasts). EC attach to the substrate, proliferate to form a confluent multilayered epithelium and can be subcultured. Using a serum-free medium (MCDB 153) EC can be grown in culture free of any undefined supplement. In organotypical culture systems, EC are seeded on a biological matrix (dermis, dermal equivalent) and lifted at the air-liquid interface. Under these conditions a high level of differentiation of the cultured epidermis can be obtained. The availability of a culture-grown epithelium, similar to the in vivo epidermis, has led to the use of cultured epidermal sheets for the treatment of wounds, in particular burns. The therapeutic results have been quite promising.
在过去的十五年中，已经开发出了各种表皮细胞（EC）培养系统，这些系统既适用于研究目的，也适用于临床应用。在器官和外植体培养中，培养皮肤组织块，在第一种情况下可获得表皮的垂直增殖，在第二种情况下可获得表皮细胞的向外迁移和增殖。在分离的表皮细胞培养中，将表皮细胞悬液接种在塑料或特定底物（胶原蛋白、纤连蛋白、3T3成纤维细胞）上。表皮细胞附着于底物，增殖形成汇合的多层上皮，并且可以进行传代培养。使用无血清培养基（MCDB 153），表皮细胞可以在无任何未定义补充物的培养条件下生长。在器官型培养系统中，将表皮细胞接种在生物基质（真皮、真皮替代物）上，并在气液界面处培养。在这些条件下，可以使培养的表皮实现高度分化。培养出的与体内表皮相似的上皮组织可用于伤口治疗，尤其是烧伤治疗。治疗结果非常令人鼓舞。
