通过透染成像测量细菌体积。

Measurement of bacterial volume by transmission-through-dye imaging.

机构信息

Department of Biological Sciences, Kent State University, Kent, OH 44242, USA.

出版信息

J Microbiol Methods. 2011 Dec;87(3):375-7. doi: 10.1016/j.mimet.2011.08.019. Epub 2011 Sep 25.

DOI:10.1016/j.mimet.2011.08.019

PMID:21982927

Abstract

Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.

摘要

传色（TTD）显微镜使得直接测量细菌体积成为可能，而无需考虑细胞形状。该技术可在任何明场显微镜上实现，适用于所有形状的细菌。TTD 成像要求完整的细菌被固定在平坦的透明表面上，如玻璃载玻片。