Darban-Sarokhalil Davood, Fooladi Abbas Ali Imani, Bameri Zakaria, Nasiri Mohammad Javad, Feizabadi Mohammad Mehdi
Tehran University of Medical Sciences, Department of Microbiology, School of Medicine, Tehran, Iran.
Acta Microbiol Immunol Hung. 2011 Sep;58(3):211-7. doi: 10.1556/AMicr.58.2011.3.4.
Cytochrome P450 CYP141 is an intermediary metabolic and respiratory protein that interferes with oxidation reduction in Mycobacterium tuberculosis. This conserved protein has also been debated as a hypothetical target for therapeutics. We used the sequences of CYP141 gene to develop a PCR for rapid detection of Mycobacterium tuberculosis from respiratory specimens. The sensitivity of this PCR for culture positive-smear positive and culture positive-smear negative samples were 92% and 62.5%, respectively. The overall sensitivity and specificity of this PCR was 85.7% and 97.8%. As compared with other studies, it appears that the CYP141 gene is a good target for direct detection of M. tuberculosis from respiratory specimens.
细胞色素P450 CYP141是一种中间代谢和呼吸蛋白，它干扰结核分枝杆菌中的氧化还原反应。这种保守蛋白也一直被作为治疗的潜在靶点进行讨论。我们利用CYP141基因序列开发了一种用于从呼吸道标本中快速检测结核分枝杆菌的聚合酶链反应（PCR）。该PCR对培养阳性-涂片阳性和培养阳性-涂片阴性样本的敏感性分别为92%和62.5%。该PCR的总体敏感性和特异性分别为85.7%和97.8%。与其他研究相比，CYP141基因似乎是从呼吸道标本中直接检测结核分枝杆菌的一个良好靶点。
