Wang Xue-qian, Zhang Hong-yan, Qi Qi-wei, Zhao Jing, Xu Li
Department of Genetics, Qingdao Research Institute for Population and Family Planning, Qingdao, Shandong, People's Republic of China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Oct;28(5):559-61. doi: 10.3760/cma.j.issn.1003-9406.2011.05.020.
To investigate the relationship between follicle stimulating hormone (FSH) and AZF microdeletion on Y chromosome.
Fifteen loci of 4 regions of the AZF gene were investigated by multiplex PCR in 100 patients with azoospermia or severe oligozoospermia. The reproductive hormone FSH was detected by access 2 immunoassay system from BECKMAN COULTER. Epidata was set up and analyzed for means. F test of anova was performed.
The rate of microdeletion was 13% (13 out of 100 patients). The deletion was on AZFa in 1 patient, AZFb+c+d in 4 patients, AZFc+d in 7 patients, AZFd in 1 patient, respectively. The level of FSH (40.8±11.3 U/L) in the AZFb+c+d deletion group was significantly higher than that in the group without Y chromosome deletion (16.7±14.3 U/L) and the other types of deletion (11.8±6.7 U/L) (P<0.01).
The common microdeletion regions were AZFc and AZFd on Y chromosome in azoospermia or severe oligozoospermia. The microdeletion of AZFb+c+d was one of the important causes of the high level of FSH.
探讨卵泡刺激素（FSH）与Y染色体上AZF微缺失之间的关系。
采用多重PCR技术对100例无精子症或严重少精子症患者的AZF基因4个区域的15个位点进行检测。采用贝克曼库尔特公司的Access 2免疫分析系统检测生殖激素FSH。建立Epidata并进行均值分析。进行方差分析的F检验。
微缺失率为13%（100例患者中有13例）。分别有1例患者的缺失发生在AZFa，4例患者的缺失发生在AZFb+c+d，7例患者的缺失发生在AZFc+d，1例患者的缺失发生在AZFd。AZFb+c+d缺失组的FSH水平（40.8±11.3 U/L）显著高于无Y染色体缺失组（16.7±14.3 U/L）和其他类型缺失组（11.8±6.7 U/L）（P<0.01）。
无精子症或严重少精子症患者Y染色体上常见的微缺失区域为AZFc和AZFd。AZFb+c+d微缺失是FSH水平升高的重要原因之一。
