Hnt3 同源蛋白的晶体结构揭示了逆转 5'-腺苷化 DNA 的机制。
Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA.
机构信息
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
出版信息
Nat Struct Mol Biol. 2011 Oct 9;18(11):1297-9. doi: 10.1038/nsmb.2145.
Aprataxin is a DNA deadenylase that resolves DNA 5'-AMP termini and reverses abortive DNA ligation. The crystal structures of Schizosaccharomyces pombe aprataxin Hnt3 in its apo form and in complex to dsDNA and dsDNA-AMP reveal how Hnt3 recognizes and processes 5'-adenylated DNA in a structure-specific manner. The bound DNA adopts a 5'-flap conformation that facilitates 5'-AMP access to the active site, where AMP cleavage occurs by a canonical catalytic mechanism.
aprataxin 是一种 DNA 去腺苷酶，可解决 DNA 5'-AMP 末端问题，并逆转无效的 DNA 连接。在其apo 形式和与 dsDNA 和 dsDNA-AMP 复合物的晶体结构中，揭示了 Schizosaccharomyces pombe aprataxin Hnt3 如何以结构特异性的方式识别和处理 5'-腺苷化 DNA。结合的 DNA 采用 5'-flap 构象，促进 5'-AMP 进入活性部位，在那里通过典型的催化机制发生 AMP 切割。
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