用于生物和生物医学应用的电子自旋共振显微镜。
ESR Microscopy for Biological and Biomedical Applications.
作者信息
Shin C S, Dunnam C R, Borbat P P, Dzikovski B, Barth E D, Halpern H J, Freed J H
机构信息
National Biomedical Center for Advanced ESR Technology, Cornell University, Ithaca, NY 14853, USA.
出版信息
Nanosci Nanotechnol Lett. 2011 Aug;3(4):561-567. doi: 10.1166/nnl.2011.1206.
We report on electron-spin resonance microscopy (ESRM) providing sub-micron resolution (~700nm) with a high spin concentration sample, i.e. lithium phthalocyanine (LiPc) crystal. For biomedical applications of our ESRM, we have imaged samples containing rat basophilic leukemia (RBL) cells as well as cancerous tissue samples with a resolution of several microns using a water soluble spin probe, Trityl_OX063_d24. Phantom samples with the nitroxide spin label, (15)N PDT, were also imaged to demonstrate that nitroxides, which are commonly used as spin labels, may also be used for ESRM applications. ESRM tissue imaging would therefore be valuable for diagnostic or therapeutic purposes. Also, ESRM can be used to study the motility or the metabolism of cells in various environments. With further modification and/or improvement of imaging probe and spectrometer instrumentation sub-micron biological images should be obtainable, thereby providing a useful tool for various biomedical applications.
我们报道了电子自旋共振显微镜（ESRM），它能对高自旋浓度样品，即酞菁锂（LiPc）晶体提供亚微米分辨率（约700纳米）。对于我们的ESRM在生物医学方面的应用，我们使用水溶性自旋探针三苯甲基_OX063_d24对含有大鼠嗜碱性白血病（RBL）细胞的样品以及癌组织样品进行了成像，分辨率达到了几微米。还对带有氮氧化物自旋标记（15）N PDT的模型样品进行了成像，以证明通常用作自旋标记的氮氧化物也可用于ESRM应用。因此，ESRM组织成像对于诊断或治疗目的将具有重要价值。此外，ESRM可用于研究各种环境中细胞的运动性或代谢。随着成像探针和光谱仪仪器的进一步改进和/或完善，应该能够获得亚微米级的生物图像，从而为各种生物医学应用提供一个有用的工具。
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