Tai Bui Huu, Trung Trinh Nam, Nhiem Nguyen Xuan, Ha Do Thi, Van Men Chu, Duong Vu Binh, Van Luong Hoang, Song Seokbean, Bae Kihwan, Kim Young Ho
College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.
J Asian Nat Prod Res. 2011 Oct;13(11):1061-8. doi: 10.1080/10286020.2011.603306. Epub 2011 Oct 10.
A new flavan-3-ol, (+)-afzelechin 5-O-β-d-glucopyranoside (2), together with 13 known flavonoids (1, 3-14), was isolated from the fruit peels of Wisteria floribunda. Their structures were assigned by detailed interpretation of NMR, MS, and CD spectroscopic data, as well as by comparing with published reports. The in vitro anti-inflammatory activity of the isolated compounds (1-14) was examined. Among them, compounds 3, 6, and 9 produced highest inhibitory effects on tumor necrosis factor alpha (TNF-α)-induced nuclear factor kappa-B activation in HepG2 cells with IC(50) values of 14.1, 16.5, and 11.9 μM, respectively. With the exception of compound 6, the compounds significantly inhibited the accumulation of pro-inflammatory inducible nitric oxide synthase and cyclooxygenase-2 proteins in TNF-α-stimulated HepG2 cells at a concentration as low as 0.1 μM.
从多花紫藤的果皮中分离出一种新的黄烷-3-醇，（+）-阿夫儿茶精5-O-β-D-吡喃葡萄糖苷（2），以及13种已知的黄酮类化合物（1, 3 - 14）。通过对核磁共振（NMR）、质谱（MS）和圆二色（CD）光谱数据的详细解析，并与已发表的报告进行比较，确定了它们的结构。对分离得到的化合物（1 - 14）的体外抗炎活性进行了研究。其中，化合物3、6和9对HepG2细胞中肿瘤坏死因子α（TNF-α）诱导的核因子κB激活具有最高的抑制作用，IC（50）值分别为14.1、16.5和11.9 μM。除化合物6外，这些化合物在低至0.1 μM的浓度下，能显著抑制TNF-α刺激的HepG2细胞中促炎诱导型一氧化氮合酶和环氧化酶-2蛋白的积累。
