Plant Chemetics lab, Chemical Genomics Centre of the Max Planck Society, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Physiol Plant. 2012 May;145(1):18-27. doi: 10.1111/j.1399-3054.2011.01528.x. Epub 2011 Nov 15.
Proteases are key players in plant development and immunity. When and where these proteases act during these processes is difficult to predict from proteomic or transcriptomic data because proteases are tightly regulated by post-translational mechanisms such as processing, phosphorylation and the presence of cofactors or inhibitors. Protease activities can be displayed using activity-based probes that react with the catalytic site of proteases in a mechanism-dependent manner. Plant proteomes have been labeled with probes for caspases, vacuolar processing enzymes, papain-like cysteine proteases, the proteasome, subtilases, prolyloligopeptidases, serine carboxypeptidases and matrix metalloproteases. Here, we review these protease probes with a focus on the specificity determinants that reside in the probe and the detection methods dictated by the reporter tag.
蛋白酶是植物发育和免疫的关键参与者。在这些过程中，蛋白酶何时以及在何处发挥作用，很难根据蛋白质组学或转录组学数据进行预测，因为蛋白酶受到翻译后机制的严格调节，如加工、磷酸化以及辅因子或抑制剂的存在。蛋白酶活性可以使用基于活性的探针来显示，这些探针以依赖于机制的方式与蛋白酶的催化位点反应。植物蛋白质组已经用半胱氨酸蛋白酶、蛋白酶体、枯草杆菌蛋白酶、脯氨酰寡肽酶、丝氨酸羧肽酶和基质金属蛋白酶的探针进行了标记。在这里，我们将重点介绍探针中存在的特异性决定因素以及报告标签决定的检测方法，对这些蛋白酶探针进行综述。
