Department of Fibre and Polymer Technology, School of Chemical Science and Engineering, Royal Institute of Technology, Stockholm, Sweden.
Acta Biomater. 2012 Jan;8(1):144-53. doi: 10.1016/j.actbio.2011.09.027. Epub 2011 Sep 28.
Electroactive degradable porous tubular scaffolds were fabricated from the blends of polycaprolactone and a hyperbranched degradable conducting copolymer at different feed ratios by a solution-casting/salt-leaching method. Scaning electron microscopy (SEM) and microcomputed tomography tests indicated that these scaffolds had homogeneously distributed interconnected pores on the cross-section and surface. The electrical conductivity of films with the same composition as the scaffolds was between 3.4×10(-6) and 3.1×10(-7) S cm(-1), depending on the ratio of hyperbranched degradable conducting copolymer to polycaprolactone. A hydrophilic surface with a contact angle of water about 30° was achieved by doping the films with (±)-10-camphorsulfonic acid. The mechanical properties of the films were investigated by tensile tests, and the morphology of the films was studied by SEM. The scaffolds were subjected to the WST test (a cell proliferation and cytotoxicity assay using water-soluble tetrazolium salts) with HaCaT keratinocyte cells, and the results show that these scaffolds are non-cytotoxic. These degradable electroactive tubular scaffolds are good candidates for neural tissue engineering application.
电活性可降解多孔管状支架由聚己内酯和超支化可降解导电共聚物在不同的进料比下通过溶液浇铸/盐析法制备而成。扫描电子显微镜（SEM）和微计算机断层扫描测试表明，这些支架在横截面和表面具有均匀分布的互联孔。具有与支架相同组成的薄膜的电导率在 3.4×10(-6) 和 3.1×10(-7) S cm(-1) 之间，这取决于超支化可降解导电共聚物与聚己内酯的比例。通过掺杂（±）-10-樟脑磺酸，可以使薄膜具有约 30°的水接触角的亲水表面。通过拉伸试验研究了薄膜的力学性能，并通过 SEM 研究了薄膜的形态。支架用 HaCaT 角质形成细胞进行 WST 试验（使用水溶性四唑盐的细胞增殖和细胞毒性测定），结果表明这些支架无细胞毒性。这些可降解的电活性管状支架是神经组织工程应用的良好候选材料。
