Department of Pathogen Biology and Immunology, Yangzhou University School of Medicine, Yangzhou, China.
J Microbiol Methods. 2011 Dec;87(3):350-4. doi: 10.1016/j.mimet.2011.09.012. Epub 2011 Oct 1.
Neisseria gonorrhoeae is the causative microorganism for the sexually transmitted disease (STD) gonorrhea and humans are its only natural host. An animal model would be a useful tool for gonorrhea research, therefore we developed the hCEACAM1 transgenic mice, using an eukaryotic expression vector, pCDPCAM1-GI. This construct was microinjected into the zygotes of C57BL/6 mice and 22 F0 generation transgenic mice were obtained. Four (lines 50, 53, 54, and 59) of the F0 generation were found to carry the transgene by PCR and sequence analysis, respectively. Western blotting and Fluorescence-Activated Cell Sorting Analysis demonstrated that hCEACAM1 was expressed on the cell membrane of various tissues in the line 53 transgenic mouse. To initiate the disease in the animal model, the F2 or F3 transgenic mice were inoculated with N. gonorrhoeae intravaginally. Compared with normal mice, N. gonorrhoeae can successfully infect and cause inflammation in the transgenic mice. These data suggested the feasibility of using hCEACAM1 transgenic mice as an animal model for gonococcal infections.
淋病奈瑟菌是性传播疾病（STD）淋病的病原体，人类是其唯一的自然宿主。动物模型将是淋病研究的有用工具，因此我们使用真核表达载体 pCDPCAM1-GI 构建了 hCEACAM1 转基因小鼠。该构建体被微注射到 C57BL/6 小鼠的受精卵中，并获得了 22 只 F0 代转基因小鼠。通过 PCR 和序列分析，发现 F0 代的 4 只（第 50、53、54 和 59 号）携带转基因。Western blot 和荧光激活细胞分选分析表明，hCEACAM1 在第 53 号转基因小鼠的各种组织的细胞膜上表达。为了在动物模型中引发疾病，F2 或 F3 代转基因小鼠经阴道接种淋病奈瑟菌。与正常小鼠相比，淋病奈瑟菌能够成功感染并引起转基因小鼠的炎症。这些数据表明，使用 hCEACAM1 转基因小鼠作为淋病感染的动物模型是可行的。
