Spencer Steven E, Valentin-Bon Iris E, Whaley Kevin, Jerse Ann E
Department of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland 20814, USA.
J Infect Dis. 2004 Feb 1;189(3):410-9. doi: 10.1086/381125. Epub 2004 Jan 20.
The development of effective vaginal microbicides is paramount in the fight against the spread of sexually transmitted infections. Preclinical testing of candidate microbicides for the prevention of gonorrhea has been seriously hindered by the lack of an animal model. We assessed the efficacy of 7 promising formulated agents--CarraGuard, Ushercell, [poly]sodium 4-styrene sulfonate (T-PSS), PRO 2000, ACIDFORM, cellulose acetate phthalate (CAP), and BufferGel--by use of a mouse model of Neisseria gonorrhoeae genital tract infection. Mice received test agent, relevant placebo, or no treatment, followed by intravaginal N. gonorrhoeae challenge. N. gonorrhoeae colonization was tested by vaginal culture. CarraGuard, Ushercell, and T-PSS demonstrated significant protection, compared with control agents and no treatment. PRO 2000, ACIDFORM, and CAP showed significant protection, compared with no treatment but not compared with respective control agents. Mice that received BufferGel were provided significant protection, compared with untreated control mice; no placebo was tested. The findings of the present study suggest that topical agents may effectively reduce N. gonorrhoeae infection and that further evaluation is warranted.
开发有效的阴道杀菌剂对于抗击性传播感染的传播至关重要。由于缺乏动物模型，用于预防淋病的候选杀菌剂的临床前测试受到严重阻碍。我们通过使用淋病奈瑟菌生殖道感染的小鼠模型，评估了7种有前景的制剂——CarraGuard、Ushercell、聚对苯乙烯磺酸钠（T-PSS）、PRO 2000、ACIDFORM、醋酸纤维素邻苯二甲酸酯（CAP）和缓冲凝胶——的疗效。小鼠接受测试制剂、相关安慰剂或不进行治疗，随后进行阴道内淋病奈瑟菌攻击。通过阴道培养检测淋病奈瑟菌定植情况。与对照制剂和不治疗相比，CarraGuard、Ushercell和T-PSS显示出显著的保护作用。与不治疗相比，PRO 2000、ACIDFORM和CAP显示出显著的保护作用，但与各自的对照制剂相比则不然。与未治疗的对照小鼠相比，接受缓冲凝胶的小鼠得到了显著的保护；未测试安慰剂。本研究结果表明，局部用药可能有效减少淋病奈瑟菌感染，值得进一步评估。
