Wen Chien-Hui, Tsao Shu-Chuan, Su Yue-Chiu, Wu Chun-Chieh, Chai Chee-Yin
Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC.
Acta Cytol. 2011;55(5):460-6. doi: 10.1159/000330674. Epub 2011 Oct 8.
The aim of this study was to find a better technique for the cell block preparation.
We developed a novel capsule-based technique and applied it to different cell block preparation materials including plasma thromboplastin (PT) clot and agarose gel-embedding medium, in order to concentrate the cells in a limited field, then compared the result with the widely used, modified alcohol fixative preparation technique based on specimen adequacy and diagnostic accuracy. Twenty-eight nongynecologic body fluids and 41 gynecologic Liqui-PREP™ (LGM International Inc., Fort Lauderdale, Fla., USA) cytology specimens were collected for cell block preparation. We performed routine hematoxylin and eosin staining on the cell block sections, which were scored according to four specimen adequacy parameters: background, cellularity, nuclear preservation and cytoplasmic preservation. The differences between the diagnostic results of cell block slide and conventional cytology smear were also presented.
The capsule-based PT clot technique in nongynecologic body fluids provided more cellularity in the cell block sections and reduced atypical diagnosis when compared to conventional smears.
We suggest the capsule-based PT clot technique is a better technique for nongynecologic body fluids in the preparation of a satisfactory cell block.
本研究旨在寻找一种更好的细胞块制备技术。
我们开发了一种基于胶囊的新技术，并将其应用于不同的细胞块制备材料，包括血浆凝血活酶（PT）凝块和琼脂糖凝胶包埋介质，以便将细胞集中在有限的视野中，然后根据标本充足率和诊断准确性，将结果与广泛使用的改良酒精固定剂制备技术进行比较。收集了28份非妇科体液和41份妇科Liqui-PREP™（LGM International Inc.，美国佛罗里达州劳德代尔堡）细胞学标本用于细胞块制备。我们对细胞块切片进行常规苏木精和伊红染色，并根据四个标本充足率参数进行评分：背景、细胞密度、核保存和胞质保存。还展示了细胞块玻片和传统细胞学涂片诊断结果之间的差异。
与传统涂片相比，基于胶囊的PT凝块技术在非妇科体液的细胞块切片中提供了更高的细胞密度，并减少了非典型诊断。
我们认为基于胶囊的PT凝块技术是一种更好的技术，用于制备令人满意的非妇科体液细胞块。
