中央杏仁核内微量注射L-精氨酸对吗啡条件化大鼠中纳洛酮诱发的位置厌恶的翻转作用。
Reversal effect of intra-central amygdala microinjection of L-arginine on place aversion induced by naloxone in morphine conditioned rats.
作者信息
Karimi Sara, Karami Manizheh, Sahraei Hedayat, Rahimpour Mahnaz
机构信息
Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
Dept. of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
出版信息
Iran Biomed J. 2011;15(3):92-9.
BACKGROUND
Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated.
METHODS
Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Naloxone (0.05-0.4 mg/kg, i.p.), a selective antagonist of mu-opioid receptor, was administered once prior to morphine response testing. NO agents were administered directly into the central amygdala (CeA) prior to naloxone injection pre-testing.
RESULTS
Morphine (2.5-10 mg/kg, s.c.) produced a significant dose-dependent place preference in experimental animals. When naloxone (0.05-0.4 mg/kg, i.p.) was injected before testing of morphine (5 mg/kg, s.c.) response, the antagonist induced a significant aversion. This response was reversed due to injection of L-arginine (0.3-3 microg/rat), intra-CeA prior to naloxone administration. However, pre-injection of L-NAME (intra-CeA), an inhibitor of NO production, blocked this effect.
CONCLUSION
The finding may reflect that NO in the nucleus participates in morphine plus naloxone interaction.
背景
先前已提出一氧化氮（NO）在仅使用经典任务的吗啡条件反射表达中的作用。在这项研究中，研究了NO在与纳洛酮注射配对的任务中对阿片类药物诱导的条件反射表达的影响。
方法
使用无偏程序在成年雄性Wistar大鼠（体重200 - 250 g）中建立条件反射。在吗啡反应测试前，腹腔注射一次纳洛酮（0.05 - 0.4 mg/kg），一种μ-阿片受体的选择性拮抗剂。在纳洛酮注射预测试前，将NO试剂直接注射到中央杏仁核（CeA）中。
结果
吗啡（2.5 - 10 mg/kg，皮下注射）在实验动物中产生了显著的剂量依赖性位置偏好。当在测试吗啡（5 mg/kg，皮下注射）反应前注射纳洛酮（0.05 - 0.4 mg/kg，腹腔注射）时，拮抗剂诱导了显著的厌恶反应。由于在纳洛酮给药前向CeA内注射L-精氨酸（0.3 - 3 μg/只大鼠），这种反应被逆转。然而，预先注射L-NAME（CeA内），一种NO产生的抑制剂，阻断了这种效应。
结论
该发现可能反映出杏仁核中的NO参与了吗啡加纳洛酮的相互作用。
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