Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China.
Synapse. 2012 Feb;66(2):151-9. doi: 10.1002/syn.20995. Epub 2011 Nov 3.
Histone deacetylases 2 (HDAC2) is expressed in the central nervous system; it has multiple functions in neural plasticity. However, we do not know if HDAC2 is also involved in the pathology of epilepsy. Here we report that HDAC2 was expressed in the brain tissues of both control and temporal lobe epilepsy (TLE) patients. Results from immunofluorescence and immunohistochemistry showed that HDAC2 was primarily located in the nucleus and that TLE patients exhibit significantly more HDAC2 positive cells than control. Western blotting showed that HDAC2 protein levels were significantly higher in TLE than in control brain. Moreover, in the rat model of TLE, there was a sustained enhancement of HDAC2 expression in rat models of TLE. HDAC2 was significantly increased in both the acute (1 day) and chronic (60 days) animals compared with control group. These results suggest that HDAC2 play an important role in the pathogenesis of human TLE.
组蛋白去乙酰化酶 2（HDAC2）在中枢神经系统中表达；它在神经可塑性中有多种功能。然而，我们不知道 HDAC2 是否也参与癫痫的病理学。在这里，我们报告 HDAC2 存在于对照和颞叶癫痫（TLE）患者的脑组织中。免疫荧光和免疫组织化学的结果表明，HDAC2 主要位于细胞核中，并且 TLE 患者的 HDAC2 阳性细胞明显多于对照。Western blot 显示 TLE 中 HDAC2 蛋白水平明显高于对照脑。此外，在 TLE 的大鼠模型中，TLE 大鼠模型中 HDAC2 的表达持续增强。与对照组相比，急性（1 天）和慢性（60 天）动物中 HDAC2 均显著增加。这些结果表明 HDAC2 在人类 TLE 的发病机制中起重要作用。
