埃博拉病毒基质蛋白 VP40 的非常规分泌。
Unconventional secretion of Ebola virus matrix protein VP40.
机构信息
Filovirus Laboratory, Inserm U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
出版信息
J Infect Dis. 2011 Nov;204 Suppl 3(Suppl 3):S833-9. doi: 10.1093/infdis/jir305.
Abstract
The Ebola virus matrix protein VP40 plays an essential role in virus assembly and budding. In this study we reveal that transient VP40 expression results in the release into the culture medium of substantial amounts of soluble monomeric VP40 in addition to the release of virus-like particles containing an oligomeric form of this protein as previously described. We show that VP40 secretion is endoplasmic reticulum/Golgi-independent and is not associated with cell death. Soluble VP40 was observed during Ebola virus infection of cells and was also found in the serum of virus-infected animals albeit in lower amounts. Unconventional secretion of VP40 may therefore play a role in Ebola virus pathogenicity.
摘要
埃博拉病毒基质蛋白 VP40 在病毒组装和出芽过程中发挥着重要作用。在这项研究中，我们揭示了瞬时表达 VP40 除了以前描述的释放含有这种蛋白多聚体形式的病毒样颗粒外，还会导致大量可溶性单体 VP40 释放到培养基中。我们表明 VP40 的分泌与内质网/高尔基体无关，也与细胞死亡无关。在埃博拉病毒感染细胞期间观察到可溶性 VP40，并且在感染病毒的动物的血清中也发现了可溶性 VP40，尽管其含量较低。因此，VP40 的非常规分泌可能在埃博拉病毒的致病性中发挥作用。
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