Macias V R, Pinzón C, Fierro F, Vergara M, Martínez D, Rosado A, Gutiérrez C G, Rosales-Torres A M
Departamento de Producción Agrícola y Animal, UAM-Xochimilco, México, D.F., México.
Reprod Domest Anim. 2012 Jun;47(3):e39-42. doi: 10.1111/j.1439-0531.2011.01919.x. Epub 2011 Oct 12.
The development of dominant follicles requires the parallel growth of a vascular network, regulated by VEGF and its receptors VEGFR-1 and VEGFR-2. Here, we demonstrate the presence of mRNA for the soluble forms of VEGFR-1 and VEGFR-2 by RT-PCR and the respective proteins by Western blot, in bovine dominant follicles. The 3' end of the mRNA coding region and the deduced C-terminal amino acid sequence of the bovine VEGFR soluble forms were similar to those previously described in human and mice. The relative abundance of sVEGFR-1 was higher in dominant follicles of day 4, decreasing on day 6 and further on day 9 of the cycle. In contrast, sVEGFR-2 expression was low on day 4 follicles and increased as the cycle advanced, becoming greater on day 9. The changes of sVEGFR-1 and sVEGFR-2 with the age of the bovine dominant follicle indicate a physiological role in its growth and atresia.
优势卵泡的发育需要血管网络的平行生长，这一过程由血管内皮生长因子（VEGF）及其受体VEGFR-1和VEGFR-2调节。在此，我们通过逆转录聚合酶链反应（RT-PCR）证明了牛优势卵泡中存在VEGFR-1和VEGFR-2可溶性形式的信使核糖核酸（mRNA），并通过蛋白质免疫印迹法检测到了相应的蛋白质。牛VEGFR可溶性形式的mRNA编码区3'末端和推导的C末端氨基酸序列与先前在人和小鼠中描述的相似。可溶性VEGFR-1（sVEGFR-1）在周期第4天的优势卵泡中相对丰度较高，在第6天下降，在第9天进一步下降。相反，sVEGFR-2在第4天卵泡中的表达较低，并随着周期进展而增加，在第9天变得更高。sVEGFR-1和sVEGFR-2随牛优势卵泡年龄的变化表明其在卵泡生长和闭锁中具有生理作用。
