Shipton C A, Marder J B, Barber J
Department of Biochemistry, Imperial College, London, U.K.
Z Naturforsch C J Biosci. 1990 May;45(5):388-94. doi: 10.1515/znc-1990-0513.
Proteolytic mapping of the D 1 subunit of photosystem two and a degradation product which arises during its rapid catabolism shows that the latter is a result of proteolysis within the peptide motif QEEET. This motif is located in a portion of the D 1 protein thought to form a stroma-exposed connection between fourth and fifth transmembrane segments. This connection domain also contains a "PEST"-like sequence and forms part of the QB/herbicide binding niche. The QEEET motif seems to provide a major epitope in immunological studies, as judged from reaction of D 1 and its fragments with polyclonal antibodies. Antibodies against D 1 were found to react with other animal and plant proteins which contain similar sequence motifs.
光系统II D1亚基的蛋白水解图谱以及其快速分解代谢过程中产生的一种降解产物表明，后者是肽基序QEEET内蛋白水解的结果。该基序位于D1蛋白的一部分中，这部分被认为在第四和第五跨膜段之间形成了一个面向基质的连接。这个连接域还包含一个“PEST”样序列，并构成了QB/除草剂结合位点的一部分。从D1及其片段与多克隆抗体的反应判断，QEEET基序似乎在免疫学研究中提供了一个主要表位。发现针对D1的抗体与其他含有相似序列基序的动植物蛋白发生反应。
