Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas & Universidad Autónoma de Madrid, E-28029 Madrid, Spain.
J Proteomics. 2012 Jan 4;75(3):783-95. doi: 10.1016/j.jprot.2011.09.016. Epub 2011 Oct 1.
Obesity is recognized as an epidemic health problem worldwide. In humans, the accumulation of omental rather than subcutaneous fat appears to be tightly linked to insulin resistance, type 2 diabetes and cardiovascular disease. Differences in gene expression profiles in the adipose tissue comparing non-obese and obese subjects have been well documented. However, to date, no comparative proteomic studies based on omental fat have investigated the influence of obesity in protein expression. In this work, we searched for proteins differentially expressed in the omental fat of non-obese and obese subjects using 2D-DIGE and MS. Forty-four proteins, several of which were further studied by immunoblotting and immunostaining analyses, showed significant differences in the expression levels in the two groups of subjects. Our findings reveal a clearly distinctive proteomic profile between obese and non-obese subjects which emphasizes: i) reduced metabolic activity in the obese fat, since most down-regulated proteins were engaged in metabolic pathways; and ii) morphological and structural cell changes in the obese fat, as revealed by the functions exerted by most up-regulated proteins. Interestingly, transketolase and aminoacylase-1 represent newly described molecules involved in the pathophysiology of obesity, thus opening up new possibilities in the study of obesity.
肥胖症已被公认为全球性的健康问题。在人类中，网膜脂肪的积累而非皮下脂肪的积累似乎与胰岛素抵抗、2 型糖尿病和心血管疾病密切相关。已有充分的文献记载，比较非肥胖和肥胖个体的脂肪组织中的基因表达谱差异。然而，迄今为止，尚无基于网膜脂肪的比较蛋白质组学研究调查肥胖对蛋白质表达的影响。在这项工作中，我们使用 2D-DIGE 和 MS 搜索非肥胖和肥胖个体网膜脂肪中差异表达的蛋白质。有 44 种蛋白质，其中一些通过免疫印迹和免疫染色分析进一步研究，它们在两组受试者中的表达水平存在显著差异。我们的发现揭示了肥胖和非肥胖个体之间明显不同的蛋白质组学特征，这强调了：i）肥胖脂肪的代谢活性降低，因为大多数下调的蛋白质参与代谢途径；ii）肥胖脂肪的形态和结构细胞变化，如大多数上调的蛋白质所发挥的功能所示。有趣的是，转酮醇酶和氨基己糖苷酶-1是新描述的与肥胖病理生理学相关的分子，这为肥胖的研究开辟了新的可能性。
