School of Chemistry and Chemical Engineering, Institution of Molecular Engineering and Applied Chemistry, Anhui University of Technology, Maanshan, 243002, People's Republic of China.
Luminescence. 2012 Sep-Oct;27(5):414-8. doi: 10.1002/bio.1365. Epub 2011 Oct 12.
Chemiluminescence (CL) of a cyclometallated iridium (III) complex {tris[1-(2,6-dimethylphenoxy)-4-(4-chlorophenyl)phthalazine]iridium(III)} in the presence of potassium permanganate and oxalic acid is reported for the first time. Cysteine exhibits sufficient enhancing effect on the CL generated from the cyclometallated iridium(III) complex, which make it possible for the sensitive detection of cysteine using a flow-injection-chemiluminescence (FI-CL) method. The optimum conditions for the chemiluminescence emission were investigated. Under the optimal condition, the linear range for the determination of cysteine was 1.0 × 10(-9) -5.0 × 10(-6) mol/L with a detection limit of 6.9 × 10(-10) mol/L. A relative standard deviation of 1.6% was obtained for eight replicate determinations. The mechanisms of CL are proposed and the emitting species was identified as the metal-to-ligand charge-transfer (MLCT) excited states of the iridium complex.
首次报道了在高锰酸钾和草酸存在下，金属铱（III）配合物[三（1-（2,6-二甲基苯氧基）-4-（4-氯苯基）酞嗪]铱（III）的化学发光（CL）。半胱氨酸对金属铱（III）配合物产生的 CL 具有足够的增强作用，这使得使用流动注射-化学发光（FI-CL）方法对半胱氨酸进行灵敏检测成为可能。研究了化学发光发射的最佳条件。在最佳条件下，测定半胱氨酸的线性范围为 1.0×10（-9）-5.0×10（-6）mol/L，检测限为 6.9×10（-10）mol/L。八个重复测定的相对标准偏差为 1.6%。提出了 CL 的机制，并确定了发射物质为铱配合物的金属-配体电荷转移（MLCT）激发态。
