Takeyama M, Wakayama K, Takayama F, Kondo K, Fujii N, Yajima H
Department of Hospital Pharmacy, Medical College of Oita, Japan.
Chem Pharm Bull (Tokyo). 1990 Apr;38(4):960-2. doi: 10.1248/cpb.38.960.
A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11-28) of porcine VIP conjugated with beta-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl beta-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was 0.1 fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100 pmol/l, which was more than eightfold higher than that in normal milk.
利用与β-D-半乳糖苷酶偶联的猪血管活性肠肽（VIP）合成羧基末端（C末端）片段（第11 - 28位氨基酸残基）和包被有二抗的免疫板，开发了一种灵敏且特异的酶免疫测定法，用于检测血管活性肠肽样免疫反应性。以4-甲基伞形酮基β-D-吡喃半乳糖苷作为荧光底物，该方法可检测到的血管活性肠肽样免疫反应性物质（VIP-IS）的最小量为0.1 fmol/孔（2.5 pmol/L）。牛乳中的VIP-IS水平高于100 pmol/L，比正常牛奶中的水平高出八倍多。
