Zuo Qiang, Dong Lele, Fan Jianjun
Department of Orthopedics, First Affiliated Hospital of Baotou Medical College, Baotou Inner Mongolia 014010, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Sep;25(9):1094-8.
To evaluate the feasibility of Seprafilm anti-adhesion membrane, a hyaluronic acid (HA) derivative, on prevention of adhesion in acute injured tendon.
Eighteen 4-month-old Chinese white rabbits (half males and half females, weighing 2.0-2.5 kg) were made the laceration models of the bilateral second and third toes of hindpaw. According to different treatments, the rabbits were randomly divided into 4 groups (n = 18). The second toe of right hindpaw was wrapped with Seprafilm anti-adhesion membrane (group A); the third toe of right hindpaw was wrapped with polylactic acid membrane (group B); the second toe of left hindpaw was coated with sodium hyaluronate gel (group C); and the third toe of left hindpaw did not treated, as control group (group D). The general condition was observed; the range of motion (ROM) of distal interphalangeal joint was measured; the gross observation and histological observation were performed at 1, 2, and 4 weeks, then the degree of adhesion was graded.
All rabbits survived to the end of the experiment. There was no significant difference in ROM of distal interphalangeal joint between groups A and B at 1, 2, and 4 weeks (P > 0.05). ROM of group A was superior to that of groups C and D at 2 and 4 weeks (P < 0.05). The gross and histological observations showed the same result in the grading of adhesion. At 1 week, there was no significant difference in the grading of adhesion among 4 groups (P > 0.05); at 2 and 4 weeks, the grading of adhesion in group A was similar to that in group B (P > 0.05), and the grading of adhesion in group A was significantly slighter than that in groups C and D (P < 0.05).
Seprafilm anti-adhesion membrane composed of HA derivative can prevent tendon adhesion and improve the joint function in acute tendon injury of rabbits.
评估透明质酸（HA）衍生物Seprafilm防粘连膜预防急性损伤肌腱粘连的可行性。
选取18只4月龄中国白兔（雌雄各半，体重2.0 - 2.5 kg），制作双侧后足第二和第三趾的切割伤模型。根据不同处理，将兔子随机分为4组（n = 18）。右后足第二趾用Seprafilm防粘连膜包裹（A组）；右后足第三趾用聚乳酸膜包裹（B组）；左后足第二趾涂抹透明质酸钠凝胶（C组）；左后足第三趾不做处理，作为对照组（D组）。观察一般情况；测量远侧指间关节活动度（ROM）；在第1、2和4周进行大体观察和组织学观察，然后对粘连程度进行分级。
所有兔子均存活至实验结束。A组和B组在第1、2和4周时远侧指间关节ROM差异无统计学意义（P > 0.05）。在第2和4周时，A组的ROM优于C组和D组（P < 0.05）。大体和组织学观察在粘连分级方面结果相同。在第1周时，4组之间粘连分级差异无统计学意义（P > 0.05）；在第2和4周时，A组粘连分级与B组相似（P > 0.05），且A组粘连分级明显轻于C组和D组（P < 0.05）。
由HA衍生物组成的Seprafilm防粘连膜可预防兔急性肌腱损伤中的肌腱粘连并改善关节功能。
