al-Mahdawi S, Shallal A, Wyse R K
Department of Paediatric Cardiology, Institute of Child Health, London, UK.
FEBS Lett. 1990 Jul 16;267(2):183-5. doi: 10.1016/0014-5793(90)80920-e.
Using Northern blot analysis and immunoblotting techniques we report for the first time, that the neural cell adhesion molecule, N-CAM, is expressed in human heart. We found several different N-CAM transcripts in human fetal (13-17 weeks gestation) and mature heart (left ventricle from a 5-year-old child). Northern blotting showed that a 5.2 kb transcript was the most abundant and progressively increased with age, both in fetal and mature heart. These transcripts may correspond with the different protein isoforms shown by immunoblotting. We also confirmed the presence of N-CAM in fetal and mature myocytes by immunohistochemical techniques, using a monoclonal antibody to human N-CAM. Results demonstrated that N-CAM is mainly confined to the myocyte cell surface.
我们首次运用Northern印迹分析和免疫印迹技术报告，神经细胞黏附分子N-CAM在人类心脏中表达。我们在人类胎儿（妊娠13 - 17周）和成熟心脏（来自一名5岁儿童的左心室）中发现了几种不同的N-CAM转录本。Northern印迹显示，一个5.2 kb的转录本最为丰富，且在胎儿和成熟心脏中均随年龄增长而逐渐增加。这些转录本可能与免疫印迹显示的不同蛋白质异构体相对应。我们还使用抗人N-CAM单克隆抗体，通过免疫组织化学技术证实了胎儿和成熟心肌细胞中存在N-CAM。结果表明，N-CAM主要局限于心肌细胞表面。
