致病性大肠杆菌和侵袭性大肠杆菌对人喉表皮样癌细胞（HEp-2细胞）侵袭能力的比较。

A comparison of HEp-2 cell invasion by enteropathogenic and enteroinvasive Escherichia coli.

作者信息

Donnenberg M S, Donohue-Rolfe A, Keusch G T

机构信息

Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Boston, MA.

出版信息

FEMS Microbiol Lett. 1990 May;57(1-2):83-6. doi: 10.1016/0378-1097(90)90417-o.

DOI:10.1016/0378-1097(90)90417-o

PMID:2199306

Abstract

In order to further characterize cellular invasion by enteropathogenic Escherichia coli (EPEC), we compared invasion of HEp-2 cells by EPEC and enteroinvasive E. coli (EIEC). We used a gentamicin HEp-2 cell assay and measured bacterial recovery under conditions of varying incubation time and temperature, and in the presence or absence of inhibitors of cellular microfilaments and microtubules. We found that, unlike EIEC, EPEC did not rapidly multiply within HEp-2 cell but invaded well at 32 degrees C. While microfilament inhibitors reduced invasion by both EIEC and EPEC, microtubule inhibitors reduced invasion by EPEC only. These results suggest that EPEC and EIEC differ in their mechanisms of epithelial cell invasion.

摘要

为了进一步描述肠致病性大肠杆菌（EPEC）的细胞侵袭特性，我们比较了EPEC和肠侵袭性大肠杆菌（EIEC）对HEp-2细胞的侵袭情况。我们采用庆大霉素处理的HEp-2细胞检测法，在不同孵育时间和温度条件下，以及在存在或不存在细胞微丝和微管抑制剂的情况下，测量细菌回收率。我们发现，与EIEC不同，EPEC在HEp-2细胞内不会快速增殖，但在32℃时侵袭能力良好。虽然微丝抑制剂可降低EIEC和EPEC的侵袭能力，但微管抑制剂仅降低EPEC的侵袭能力。这些结果表明，EPEC和EIEC在上皮细胞侵袭机制上存在差异。