Johnson D I, O'Brien J M, Jacobs C W
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405.
Gene. 1990 May 31;90(1):93-8. doi: 10.1016/0378-1119(90)90443-u.
The Saccharomyces cerevisiae CDC43 gene product is involved in establishing cell polarity during the cell-division cycle. When grown at restrictive temperatures, temperature-sensitive cdc43 mutants are unable to form buds and display delocalized cell-surface deposition [Adams et al., J. Cell Biol. (1990) in press]. We have isolated a cdc43-complementing plasmid from a yeast genomic-DNA library and localized the CDC43 gene, by subcloning and transposon-mutagenesis experiments, to a 1.2-kb region of DNA that contained only one significant ATG-initiated open reading frame of 213 codons. The putative CDC43 gene product contains a possible nuclear-localization signal sequence, a cysteine-rich domain and a histidine-rich domain, and a region that is similar in structure to alpha-helix-turn-alpha-helix structural domains present in some prokaryotic and eukaryotic DNA-binding proteins.
酿酒酵母CDC43基因产物在细胞分裂周期中参与建立细胞极性。当在限制温度下生长时，温度敏感型cdc43突变体无法形成芽，并表现出细胞表面沉积的定位异常[亚当斯等人，《细胞生物学杂志》（1990年）即将发表]。我们从酵母基因组DNA文库中分离出一个能互补cdc43的质粒，并通过亚克隆和转座子诱变实验，将CDC43基因定位到一段1.2 kb的DNA区域，该区域仅包含一个由213个密码子组成的重要的ATG起始开放阅读框。推测的CDC43基因产物包含一个可能的核定位信号序列、一个富含半胱氨酸的结构域和一个富含组氨酸的结构域，以及一个在结构上与某些原核和真核DNA结合蛋白中存在的α-螺旋-转角-α-螺旋结构域相似的区域。
