分析氨基酸单体形成的循环热解产物。

Analysis of cyclic pyrolysis products formed from amino acid monomer.

机构信息

Department of Chemistry, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, South Korea.

出版信息

J Chromatogr A. 2011 Nov 18;1218(46):8443-55. doi: 10.1016/j.chroma.2011.09.055. Epub 2011 Sep 24.

DOI:10.1016/j.chroma.2011.09.055

PMID:21993510

Abstract

Amino acid was mixed with silica and tetramethylammonium hydroxide (TMAH) to favor pyrolysis of amino acid monomer. The pyrolysis products formed from amino acid monomer were using GC/MS and GC. 20 amino acids of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were analyzed. The pyrolysis products were divided into cyclic and non-cyclic products. Among the 20 amino acids, arginine, asparagine, glutamic acid, glutamine, histidine, lysine, and phenylalanine generated cyclic pyrolysis products of the monomer. New cyclic pyrolysis products were formed by isolation of amino acid monomers. They commonly had polar side functional groups to 5-, 6-, or 7-membered ring structure. Arginine, asparagine, glutamic acid, glutamine, histidine, and phenylalanine generated only 5- or 6-membered ring products. However, lysine generated both 6- and 7-membered ring compounds. Variations of the relative intensities of the cyclic pyrolysis products with the pyrolysis temperature and amino acid concentration were also investigated.

摘要

氨基酸与二氧化硅和四甲基氢氧化铵（TMAH）混合，有利于氨基酸单体的热解。使用 GC/MS 和 GC 分析了由氨基酸单体形成的热解产物。分析了丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、脯氨酸、丝氨酸、苏氨酸、色氨酸、酪氨酸和缬氨酸这 20 种氨基酸。热解产物分为环状和非环状产物。在这 20 种氨基酸中，精氨酸、天冬酰胺、谷氨酸、谷氨酰胺、组氨酸、赖氨酸和苯丙氨酸生成了单体的环状热解产物。通过分离氨基酸单体形成了新的环状热解产物。它们通常具有极性侧官能团，形成 5-、6-或 7-元环结构。精氨酸、天冬酰胺、谷氨酸、谷氨酰胺、组氨酸和苯丙氨酸只生成 5-或 6-元环产物。然而，赖氨酸生成了 6-和 7-元环化合物。还研究了环状热解产物的相对强度随热解温度和氨基酸浓度的变化。