Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Jena, Germany.
J Chromatogr A. 2011 Nov 18;1218(46):8370-8. doi: 10.1016/j.chroma.2011.09.044. Epub 2011 Sep 22.
In this study liquid chromatography at critical conditions for poly(2-ethyl-2-oxazoline)s (PEtOx) has been performed for the first time in order to analyze functional PEtOx homopolymers and block copolymers. Besides the verification of the critical point of adsorption with two series of ester end group functionalized PEtOx homopolymers, to evaluate the effect of both the chain length dependence and the end group polarity, using a cyano column with a solvent combination of 2-propanol and water, also two-dimensional liquid chromatography (2D-LC) has been applied for a poly(2-oxazoline) block copolymer. The combined characterization techniques provided further information about the polymerization procedure with regard to the formation of side-products by separation of the block copolymer from the corresponding homopolymer impurities. In addition, hyphenation of LCCC with MALDI-TOF MS and ESI-Q-TOF tandem mass spectrometry verified the obtained results.
本研究首次在临界条件下对聚（2-乙基-2-恶唑啉）（PEtOx）进行了液相色谱分析，旨在分析功能性的 PEtOx 均聚物和嵌段共聚物。除了用两种酯端基官能化的 PEtOx 均聚物验证吸附临界点外，还使用氰基柱和 2-丙醇和水的溶剂组合，评估了链长依赖性和端基极性的影响，也对聚（2-恶唑啉）嵌段共聚物进行了二维液相色谱（2D-LC）分析。组合的表征技术通过从相应的均聚物杂质中分离嵌段共聚物，提供了关于聚合过程的进一步信息，包括副产物的形成。此外，将 LCCC 与 MALDI-TOF MS 和 ESI-Q-TOF 串联质谱联用，验证了所得到的结果。
