National Chromatographic R&A Center, CAS Key Laboratory of Separation of Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
J Chromatogr A. 2012 Mar 9;1228:193-204. doi: 10.1016/j.chroma.2011.09.051. Epub 2011 Sep 22.
Sample preparation has been playing an important role in the analysis of complex samples. Mesoporous materials as the promising adsorbents have gained increasing research interest in sample preparation due to their desirable characteristics of high surface area, large pore volume, tunable mesoporous channels with well defined pore-size distribution, controllable wall composition, as well as modifiable surface properties. The aim of this paper is to review the recent advances of mesoporous materials in sample preparation with emphases on extraction of metal ions, adsorption of organic compounds, size selective enrichment of peptides/proteins, specific capture of post-translational peptides/proteins and enzymatic reactor for protein digestion.
样品前处理在复杂样品分析中起着重要作用。介孔材料作为有前途的吸附剂,由于其具有高比表面积、大孔体积、可调介孔通道、具有良好定义的孔径分布、可控壁组成以及可修饰的表面性质等理想特性,在样品前处理中引起了越来越多的研究兴趣。本文旨在综述介孔材料在样品前处理中的最新进展,重点介绍金属离子的萃取、有机化合物的吸附、肽/蛋白质的选择性富集、翻译后肽/蛋白质的特异性捕获以及蛋白质消化的酶反应器。