Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Macromol Biosci. 2012 Jan;12(1):67-74. doi: 10.1002/mabi.201100125. Epub 2011 Oct 12.
Polylaminin (polyLM) is a polymerized form of the extracellular matrix protein laminin obtained upon pH acidification. Here microscopy and spectroscopic tools are used to study the cell compatibility and the structural stability of polyLM, aiming at establishing its robustness as a biopolymer for therapeutic use. PolyLM is cell compatible as judged by the efficiency of attachment and neuritogenesis. It is resistant to low temperature. Addition of urea or an increase in hydrostatic pressure leads to polymer disassembly. PolyLM biofilms remain stable for 48 h in contact with cell culture medium. The sedimented polymer recovered after centrifugation and adsorbed on a glass coverslip preserved its original structure and its biological properties.
多聚层粘连蛋白（polyLM）是细胞外基质蛋白层粘连蛋白在 pH 酸化时聚合的产物。本研究采用显微镜和光谱技术研究了 polyLM 的细胞相容性和结构稳定性，旨在确定其作为治疗用生物聚合物的稳定性。polyLM 对细胞具有相容性，这可从细胞黏附和神经突生成的效率判断。polyLM 能够耐受低温。加入尿素或增加静水压力会导致聚合物解聚。polyLM 生物膜在与细胞培养基接触 48 小时内保持稳定。经离心沉淀并吸附在玻璃盖玻片上的聚合物仍保留其原始结构和生物学特性。
