一种用于检测甲型流感病毒和新型甲型 H1N1 流感病毒的新型双重实时逆转录聚合酶链反应检测方法。
A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain.
机构信息
Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia; E-Mails:
出版信息
Viruses. 2009 Dec;1(3):1204-8. doi: 10.3390/v1031204. Epub 2009 Dec 9.
Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.
及时实施抗病毒治疗和其他基于公共卫生的措施取决于对新型甲型 H1N1 流感病毒的准确、快速诊断。在这项研究中，我们开发了一种用于同时检测广泛的流感 A 亚型和特定检测新型 H1N1 2009 大流行株的双重实时 PCR（RT-PCR）（dFLU-TM）检测方法。该检测方法与使用 183 份临床样本和 10 份季节性流感 A 分离株的两种先前描述的单重 RT-PCR 检测方法的综合结果进行了比较。总体而言，结果表明，dFLU-TM RT-PCR 方法适用于临床样本中流感 A（包括新型 H1N1 大流行株）的检测。
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