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大鼠肝移植后嵌合现象与急性移植物抗宿主病的相关性

Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation.

作者信息

Xue Fei, Chen Wei, Bai Xue-Li, Xu Guo-Dong, Liang Liang, Liang Ting-Bo

机构信息

Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.

出版信息

Int J Hepatol. 2011;2011:947150. doi: 10.4061/2011/947150. Epub 2011 May 17.

DOI:10.4061/2011/947150
PMID:21994878
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3170856/
Abstract

The accurate diagnosis of acute graft-versus-host disease following liver transplantation (LTx-aGVHD) has been hampered. Chimerism appears in the majority of recipients after LT and its significance in the diagnosis of LTx-aGVHD has not been clearly established. To demonstrate the significance of chimerism on the diagnosis of LTx-aGVHD, we compared the change of chimerism in syngeneic LT recipients, semiallogeneic LT recipients, and LTx-aGVHD induced recipients. Chimerism in PBMCs following sex-mismatched LT was identified by real-time PCR based on a rat Y-chromosome-specific primer. All recipients in semiallogeneic group grew in a normal pattern. However, when 4 × 10(8) donor splenocytes were transferred simultaneously during LT, the morbidity of lethal aGVHD was 100%. The chimerism appeared slightly higher in the semiallogeneic group than in the syngeneic LT group, but the difference was not significant. However, when the recipients developed lethal aGVHD after LT, chimerism in the PBMCs increased progressively, and even at an early time, a significant increase in chimerism was observed. In conclusion, high level chimerism correlated well with LTx-aGVHD, and detection of chimerism soon after transplantation may be of value in the diagnosis of LTx-aGVHD prior to the onset of symptoms.

摘要

肝移植后急性移植物抗宿主病（LTx-aGVHD）的准确诊断受到了阻碍。嵌合体在大多数肝移植受者中出现，其在LTx-aGVHD诊断中的意义尚未明确确立。为了证明嵌合体在LTx-aGVHD诊断中的意义，我们比较了同基因肝移植受者、半同种异体肝移植受者和诱导产生LTx-aGVHD的受者中嵌合体的变化。基于大鼠Y染色体特异性引物，通过实时PCR鉴定性别不匹配肝移植后外周血单个核细胞中的嵌合体。半同种异体组中的所有受者均正常生长。然而，当在肝移植期间同时输注4×10⁸个供体脾细胞时，致死性aGVHD的发病率为100%。半同种异体组中的嵌合体出现率略高于同基因肝移植组，但差异不显著。然而，当受者在肝移植后发生致死性aGVHD时，外周血单个核细胞中的嵌合体逐渐增加，甚至在早期，嵌合体就出现了显著增加。总之，高水平嵌合体与LTx-aGVHD密切相关，移植后不久检测嵌合体可能在症状出现前的LTx-aGVHD诊断中具有价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/e0a9cffe3925/IJHEP2011-947150.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/8d2a5886c770/IJHEP2011-947150.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/da8957424e71/IJHEP2011-947150.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/55b93bd01164/IJHEP2011-947150.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/26853f446f30/IJHEP2011-947150.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/e0a9cffe3925/IJHEP2011-947150.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/8d2a5886c770/IJHEP2011-947150.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/da8957424e71/IJHEP2011-947150.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/55b93bd01164/IJHEP2011-947150.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/26853f446f30/IJHEP2011-947150.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7db2/3170856/e0a9cffe3925/IJHEP2011-947150.005.jpg

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Regulatory T cells contribute to the immunoregulatory effect on graft versus host reaction after liver transplantation in donor-dominant one-way MHC matching rats.在供体主导的单向主要组织相容性复合体匹配大鼠肝移植后，调节性T细胞对移植物抗宿主反应发挥免疫调节作用。
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Graft vs. host disease after liver transplantation: a new approach is needed.
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Liver Transpl. 2007 Aug;13(8):1092-9. doi: 10.1002/lt.21203.
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Recipient and donor factors influence the incidence of graft-vs.-host disease in liver transplant patients.受者和供者因素影响肝移植患者移植物抗宿主病的发生率。
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