固定延迟是否会影响处理后组织中的有丝分裂象数量？
Does delay in fixation affect the number of mitotic figures in processed tissue?
作者信息
Cross S S, Start R D, Smith J H
机构信息
Department of Histopathology, Northern General Hospital, Sheffield.
出版信息
J Clin Pathol. 1990 Jul;43(7):597-9. doi: 10.1136/jcp.43.7.597.
Abstract
The effect of delay in fixation on the number of mitotic figures in tissues has received little attention, and previous studies have reached differing conclusions. The numbers of mitotic figures in the normal mucosa of six colectomy specimens were counted with delays in fixation of 30 minutes, one hour, two hours, three hours and six hours for samples from each specimen. The numbers of mitotic figures were counted in 50 whole crypts in each specimen by two observers. All phases of mitosis were counted. The number of observable mitotic figures declined by about 30% with a delay in fixation of two hours and by 50% with a delay of six hours. This observation has important implications for the handling of surgical specimens.
摘要
固定延迟对组织中有丝分裂相数量的影响鲜受关注，先前的研究得出了不同的结论。对六个结肠切除术标本的正常黏膜进行有丝分裂相计数，每个标本的样本分别延迟固定30分钟、1小时、2小时、3小时和6小时。两名观察者对每个标本的50个完整隐窝中的有丝分裂相进行计数。统计有丝分裂的所有阶段。固定延迟2小时，可观察到的有丝分裂相数量减少约30%；延迟6小时，减少50%。这一观察结果对外科手术标本的处理具有重要意义。
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