Department of Chemistry, Renmin University of China, Beijing 100872, PR China.
Langmuir. 2012 Jan 31;28(4):1966-70. doi: 10.1021/la203151b. Epub 2011 Oct 26.
We report a general approach to bimodify gold nanoparticles (GNPs) with two different DNA strands via DNA template reaction. Two thioctic acid modified DNA strands, one at 5' end and one at 3' end, were attached to GNPs through bivalent thiol-gold bond. By sequence design, assemblies of 5 nm GNPs chains, 10 nm GNPs chains and alternative arrangement of 5 and 10 nm GNPs could be achieved. Gel electrophoresis, transmission electron microscope (TEM), UV-vis spectra were used to characterize the assemblies. It is believed that this new kind of bimodified GNPs with two different DNA strands at different ends would enrich the toolbox of DNA-GNP conjugates and provide diverse selectivity for further assembly.
我们报告了一种通过 DNA 模板反应将两种不同的 DNA 链双修饰金纳米粒子（GNPs）的通用方法。两条巯基乙酸修饰的 DNA 链，一条在 5'端，一条在 3'端，通过二价硫醇-金键连接到 GNPs 上。通过序列设计，可以实现 5nm GNPs 链、10nm GNPs 链的组装以及 5nm 和 10nm GNPs 的交替排列。凝胶电泳、透射电子显微镜（TEM）、紫外可见光谱用于表征组装体。相信这种具有两种不同 DNA 链在不同末端的新型双修饰 GNPs 将丰富 DNA-GNP 缀合物的工具包，并为进一步组装提供多种选择性。
