a National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Genome. 2011 Nov;54(11):954-8. doi: 10.1139/g11-050. Epub 2011 Oct 13.
MicroRNAs (miRNAs), a group of small noncoding RNAs, may serve as a class of post-transcriptional regulators in plant immune systems. Nevertheless, little is known about their roles in plant immune response to the oomycete pathogens. To identify miRNAs involved in the response of soybean to Phytophthora sojae, we examined expressional patterns of miRNAs upon infection by P. sojae by microarray analysis in three soybean cultivars: Williams (susceptible), Conrad (quantitative resistance), and Williams 82 (qualitative resistance). Expression of a number of miRNAs was significantly altered upon infection and (or) in the different genotypes. qRT-PCR data with some miRNAs further confirmed the microarray results. Comparative analysis of the selected miRNAs and their targeted gene expression datasets uncovered many reciprocally expressed miRNA-target pairs, which could proposed a feedback circuit between miRNA(s) and protein-coding genes. These results may serve as a basis for further in-depth studies of miRNAs involved in soybean resistance to P. sojae.
MicroRNAs (miRNAs)，一组小型非编码 RNA，可以作为植物免疫系统中的一类转录后调控因子。然而，对于它们在植物对卵菌病原体的免疫反应中的作用知之甚少。为了鉴定参与大豆对大豆疫霉反应的 miRNAs，我们通过微阵列分析在三个大豆品种中检查了 P. sojae 感染后 miRNAs 的表达模式：Williams（易感）、Conrad（定量抗性）和 Williams 82（定性抗性）。感染和（或）在不同基因型中，许多 miRNAs 的表达发生了显著改变。一些 miRNAs 的 qRT-PCR 数据进一步证实了微阵列结果。对选定的 miRNAs 及其靶向基因表达数据集的比较分析揭示了许多相互表达的 miRNA-靶对，这可能提出了 miRNA(s) 和蛋白质编码基因之间的反馈回路。这些结果可以为进一步深入研究参与大豆对 P. sojae 抗性的 miRNAs 提供基础。
