de Walthoffen S Walter, Mlynarczyk A, Sawicka-Grzelak A, Durlik M, Paczek L, Chmura A, Ciszek M, Chabros L, Baczkowska T, Mlynarczyk G
Department of Medical Microbiology, the Medical University of Warsaw, Poland.
Transplant Proc. 2011 Oct;43(8):3128-9. doi: 10.1016/j.transproceed.2011.08.003.
The aims of this work were to define the effectiveness of identification of the extended-spectrum beta-lactamases (ESBL) phenotype, and to define the genotype of Klebsiella pneumoniae β-lactamase.
We identified ESBL phenotypes in 110 strains of K pneumoniae isolated from samples from patients of transplantation wards, using the double-disk synergy test (DDST). For the chosen strains, polymerase chain reaction (PCR) was applied to detect genes determining SHV, CTX-M, and TEM.
We showed synergism of clavulanic acid and investigated antibiotics including ceftazidime (89.1%), cefotaxime (80%), and aztreonam (82.7%) against ESBL-positive strains PCR revealed that TEM and CTX-M were present in 88.89% of strains.
The ESBL mechanism of resistance is frequent among K pneumoniae strains isolated from transplant recipients. Strains with simultaneous synthesis of more than one beta-lactamase predominated.
本研究旨在确定超广谱β-内酰胺酶（ESBL）表型鉴定的有效性，并确定肺炎克雷伯菌β-内酰胺酶的基因型。
我们使用双纸片协同试验（DDST），从移植病房患者样本中分离出的110株肺炎克雷伯菌中鉴定ESBL表型。对于所选菌株，应用聚合酶链反应（PCR）检测决定SHV、CTX-M和TEM的基因。
我们发现克拉维酸与包括头孢他啶（89.1%）、头孢噻肟（80%）和氨曲南（82.7%）在内的受试抗生素对ESBL阳性菌株具有协同作用。PCR显示，88.89%的菌株中存在TEM和CTX-M。
在从移植受者中分离出的肺炎克雷伯菌菌株中，ESBL耐药机制很常见。同时合成多种β-内酰胺酶的菌株占主导地位。
