Department of Microbiology, Peking University Health Sciences, Beijing, China.
Clin Immunol. 2011 Dec;141(3):268-72. doi: 10.1016/j.clim.2011.09.003. Epub 2011 Sep 23.
The invariant NKT cells are involved in both immunity and immune tolerance. However, their roles in transplant models remain controversial. We studied the role of NKT cells in the allograft response using two different strains of NKT deficient mice (CD1d-/- and Jα18-/- mice), and found that CD1d-/- and Jα18-/- mice rejected islet allografts with a similar kinetics as wild type B6 mice. Treatment of CD1d-/- and Jα18-/- mice with donor specific transfusion and anti-CD154 induced donor specific tolerance, which was identical to similarly treated wt B6 mice. The islet allograft tolerance requires Foxp3(+) Tregs. In the periphery, Foxp3(+) Tregs in CD1d-/-, Jα18-/-, and wt B6 mice were comparable both phenotypically and functionally. In addition, CD1d-/- and Jα18-/- CD4(+) T cells (non-Tregs) could be readily converted to Foxp3(+) Tregs by TGF-β in vitro. Our data suggest that islet allograft tolerance can be successfully established without invariant NKT cells.
固有自然杀伤 T（iNKT）细胞参与免疫和免疫耐受。然而，它们在移植模型中的作用仍存在争议。我们使用两种不同的 iNKT 缺陷小鼠（CD1d-/- 和 Jα18-/- 小鼠）研究了 iNKT 细胞在同种异体移植物反应中的作用，发现 CD1d-/- 和 Jα18-/- 小鼠与野生型 B6 小鼠具有相似的动力学排斥胰岛同种异体移植物。用供体特异性输血和抗 CD154 治疗 CD1d-/- 和 Jα18-/- 小鼠可诱导供体特异性耐受，与类似治疗的 wt B6 小鼠相同。胰岛同种异体移植物耐受需要 Foxp3(+)Treg。在外周血中，CD1d-/-、Jα18-/-和 wt B6 小鼠的 Foxp3(+)Treg 在表型和功能上均相似。此外，CD1d-/-和 Jα18-/-CD4(+)T 细胞（非 Treg）可在体外通过 TGF-β 轻易转化为 Foxp3(+)Treg。我们的数据表明，在没有固有自然杀伤 T 细胞的情况下，可以成功建立胰岛同种异体移植物耐受。
