Department of Ophthalmology, Geneva University Hospitals, Geneva, Switzerland.
Cornea. 2011 Dec;30(12):1461-4. doi: 10.1097/ICO.0b013e31821821fe.
To set up a culture assay of chick corneal endothelial cells (CECs) for transplantation into host corneas.
Histology sections were performed at 6, 9, 12, 15, and 18 embryonic days of development of the chick embryo. Visualization of the gross morphology of CECs and of epithelium, stroma, and Descemet membrane was performed. Transplantation of CECs at 18 embryonic days of development into explanted, denuded from endothelial cell, host corneas of the same stage was attempted.
The results from the histological sections clearly indicate that after embryonic day 12, the endothelial cells are well differentiated and the proliferation is complete. Transplanted CECs were able to migrate and integrate into the denuded host corneas.
This study demonstrated its feasibility using an easy accessible model of chick cornea. With this technique, sufficient CECs may be obtained for biochemical and functional investigations using only nonhatched chickens that are easily accessible and easy to manipulate.
建立鸡角膜内皮细胞（CEC）的培养方法，用于移植到宿主角膜中。
在鸡胚发育的第 6、9、12、15 和 18 天进行组织学切片。观察 CECs 以及上皮、基质和 Descemet 膜的大体形态。尝试将第 18 天发育的 CEC 移植到同一阶段的离体、去内皮细胞的宿主角膜中。
组织学切片的结果清楚地表明，在胚胎第 12 天之后，内皮细胞分化良好，增殖完全。移植的 CEC 能够迁移并整合到去内皮的宿主角膜中。
本研究使用鸡角膜这一易于获取的模型证明了其可行性。通过这种技术，仅使用易于获取和操作的未孵化的鸡即可获得足够的 CEC 进行生化和功能研究。
