Honda T
Research Institute for Microbial Diseases, Osaka University, Suita.
Rinsho Byori. 1990 May;38(5):477-80.
Several newly developed methods, including immunological and DNA hybridization techniques, have been recently introduced in clinical diagnosis. Target molecules for these detection methods are either avirulent materials or virulence factors specific to the individual causative pathogen. Examples requiring the detection of bacterial virulence factors for the accurate identification of causative agents are increasing; for example differentiation of enterotoxigenic Escherichia coli from nonpathogenic E. coli, and identification of cholera toxin-producing (or non-producing) V. cholerae 01. Recent progress in the detection of virulence factors is described. Advantages and disadvantages of various detection methods of bacterial virulence factors are also discussed.
包括免疫和DNA杂交技术在内的几种新开发的方法最近已被引入临床诊断。这些检测方法的靶分子要么是无毒物质，要么是特定致病病原体特有的毒力因子。需要检测细菌毒力因子以准确鉴定病原体的例子越来越多；例如，产肠毒素大肠杆菌与非致病性大肠杆菌的区分，以及产霍乱毒素（或不产霍乱毒素）的霍乱弧菌O1的鉴定。本文描述了毒力因子检测方面的最新进展。还讨论了细菌毒力因子各种检测方法的优缺点。
