Department of Chemistry, University of Basel, Basel, Switzerland.
Electrophoresis. 2011 Nov;32(21):3000-7. doi: 10.1002/elps.201100200. Epub 2011 Oct 14.
It is demonstrated that a hydrodynamic flow superimposed on the mobility of analyte anions can be used for the optimization of analysis time in capillary zone electrophoresis. It was also possible to use the approach for counter-balancing the electroosmotic flow and this works as well as the use of surface modifiers. To avoid any band-broadening due to the bulk flow narrow capillaries of 10 μm internal diameter were employed. This was enabled by the use of capacitively coupled contactless conductivity detection, which does not suffer from the downscaling, and detection down to between 1 and 20 μM for a range of inorganic and small organic anions was found feasible. Precisely controlled hydrodynamic flow was generated with a sequential injection manifold based on a syringe pump. Sample injection was carried out with a new design relying on a simple piece of capillary tubing to achieve the appropriate back-pressure for the required split-injection procedure.
研究表明，在分析物阴离子的迁移率上叠加一个流体动力学流可以用于优化毛细管区带电泳的分析时间。该方法还可以用于平衡电渗流，其效果与使用表面修饰剂相当。为了避免由于主体流动而导致的带展宽，使用了 10 μm 内径的窄毛细管。这是通过使用容性耦合非接触式电导检测来实现的，该检测方法不会受到缩尺的影响，并且对于一系列无机和小分子阴离子，检测下限可以达到 1 到 20 μM。通过基于注射器泵的顺序注射阀，精确控制了流体动力学流。样品注入采用了一种新的设计，依赖于一段简单的毛细管来实现所需的分流注入程序所需的回压。
