一种荧光脂肪酸探针 DAUDA，选择性置换人血清白蛋白结合的两个豆蔻酸。
A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin.
机构信息
State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China.
出版信息
Protein Sci. 2011 Dec;20(12):2095-101. doi: 10.1002/pro.749. Epub 2011 Nov 9.
Abstract
11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.
摘要
11-(Dansylamino)十一酸（DAUDA）是一种丹磺酰基荧光团，已广泛用作测定人血清白蛋白（HSA）结合部位的探针。在这里，我们报道了 HSA-豆蔻酸-DAUDA 三元复合物的结构，并清楚地确定了两个 DAUDA 分子位于 HSA 的脂肪酸（FA）结合位点 6 和 7，从而表明这两个位点是弱 FA 结合位点。该结果还表明，DAUDA 是先前研究中 HSA 上 FA 位点 6 和 7 的合适探针，但不是 HSA 上可能是胆红素结合位点的 FA 结合位点 1 的良好探针。
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