类固醇作用下的 P450（BM3）：这种瑞士军刀式的 P450 酶变得更强大了。
P450(BM3) on steroids: the Swiss Army knife P450 enzyme just gets better.
机构信息
Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, UK.
出版信息
Chembiochem. 2011 Nov 25;12(17):2537-9. doi: 10.1002/cbic.201100606. Epub 2011 Oct 13.
PMID:21998091
Abstract
The catalytic regio- and stereoselective oxidation of aliphatic C-H bonds by using atmospheric dioxygen as the oxidant is a highly desirable reaction. Reetz and co-workers have recently reported the development of P450(BM3) mutants that oxidise testosterone with greatly increased turnover activity, total stereoselectivity and >95 % regioselectivity.
摘要
使用大气中的氧气作为氧化剂，对脂肪族 C-H 键进行催化区域和立体选择性氧化是一种非常理想的反应。Reetz 及其同事最近报道了 P450(BM3)突变体的开发，这些突变体可大大提高睾酮的转化率活性、总立体选择性和 >95%的区域选择性。
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