State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.
PLoS One. 2011;6(10):e25596. doi: 10.1371/journal.pone.0025596. Epub 2011 Oct 5.
Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.
植物乳杆菌是一种广泛存在的益生菌，存在于许多发酵食品中。在本研究中，采用二维电泳法分离了植物乳杆菌的全细胞蛋白和分泌蛋白。通过串联质谱鉴定了 434 种蛋白质，包括质粒编码的假设蛋白 pLP9000_05。列出了前 20 种丰度最高的蛋白质的信息，以便对植物乳杆菌进行进一步的遗传操作，如构建高水平表达系统。此外，还通过 Blue-Native/SDS-PAGE 技术建立了植物乳杆菌的第一个相互作用图谱。同时鉴定出由麦芽糖磷酸化酶 Map3 和 Map2 组成的异二聚体复合物，以及由 Map3 和 Map2 分别组成的两个同二聚体复合物，表明这些蛋白质具有重要作用。这些发现为植物乳杆菌的进一步蛋白质组学研究提供了有价值的信息。
